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عنوان انگلیسی Construction of a Genetic Linkage Map to Locate QTLS Controlling Pathotype-Specific Powdery Mildew Resistance in Diploid Roses
چکیده انگلیسی مقاله Disease resistance is a sought-after trait in plant breeding programmes. One strategy to make resistance more durable is to increase the number of resistance genes, thereby increasing the number of pathotypes withstood. A diploid rose population (90 genotypes) derived from a cross between R. wichurana and Rosa ‘Yesterday’ was used to construct a genetic linkage map encompassing 20 AFLP primer combinations, 43 SSR, and 2 morphological markers. By applying the F1 pseudo test cross population strategy, 2 parental linkage maps were constructed (parent ‘Yesterday’ 536 cM; parent R. wichurana 526 cM). Both parental maps consisted of 7 linkage groups with an average length of 70 cM (Kosambi) corresponding to the 7 haploid rose chromosomes. These new maps were used to identify QTLs controlling disease resistance. The offspring population was screened for resistance to 2 powdery mildew pathotypes, R-E and R-P. QTLs for controlling pathotype-specific disease resistance were mapped by applying Kruskal-Wallis rank-sum tests and simple interval mapping. With 2 pathotypes analysed, 9 QTL loci were detected on linkage groups 2, 3, 5 and 6, explaining 15-73% of the phenotypic variance for pathotype-specific disease response. The genetic maps developed here will be useful for future rose breeding, pathotype-specific resistance research and development of a consensus map for roses.
کلیدواژه‌های انگلیسی مقاله disease resistance, SSR, Molecular markers, AFLP, QTL mapping, R. wichurana, 'Yesterday'

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نشانی اینترنتی http://www.irhc2017.org
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