| چکیده انگلیسی مقاله |
ABSTRACT Infertility is accepted as an essential health problem which vexes one per 6 couples. World Health Organization (WHO) defined it as the inability to succeed in a clinical pregnancy for 12 months or more of regular unprotected intercourse. In Iran, a meta-analysis has calculated infertility proportion of 10.9% [1]. In this work, the ATR–FTIR spectra of 16 embryo culture medium samples in IVF cycles, including 8 positive and 8 negative pregnancy results were compared and evaluated using several statistical methods such as cluster analysis and principal component analysis (PCA). Clinical pregnancy was validated by analyzing the serum β-Hcg levels, which was quantitatively examined after embryo transfer (ET) about 15 days later, in Imam Khomeini Hospital, Tehran, Iran (associated with Tehran University of Medical Sciences). Most signals in this region are related to carbohydrates and amino acids structure, at 1375–1385 cm−1 and 1455 cm−1. In addition, these signals are related to the C-H bending vibrations (symmetric (sym)) in CH3 and C-H scissoring in alicyclic -CH2, respectively. The band at 1635 cm−1 is attributed to amide I due to C-Ostretching vibration, which is a characteristic absorption band of β-sheet in proteins and amino acids structure. The band at 2920 cm−1 apportioned with CH2 stretching vibration of lipids, long-chain fatty acids and the band at 2952 cm−1 assigned to CH3 stretching vibration of lipids, cholesterol esters, and fatty acids [2]. Principal component analysis (PCA) method was successfully applied to mid-IR spectra as outlier detection by application of Hotelling T2 statistic procedure. Among the 1141 wavenumbers, 18 wavenumbers are selected which include the most spectral information by successive projections algorithm. The successive projections algorithm - sum of ranking differences approach for classification of the embryo culture medium samples exhibited the best performance and obtained accuracy, error rates, and non-error rate of 90%, 6% and 94% in the test set. Hence, the proposed method is rapid, simple and accurate for e discrimination of the embryo culture medium samples based on their morphology results. |
