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هفتمین همایش بیو انفورماتیک ایران
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
In silico prediction of substrate specificity of adenylation domains of NRPSs genes of some cyanobacteria strains as a mining tool for natural bioactive compounds |
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| چکیده انگلیسی مقاله |
Cyanobacteria have the ability to produce a wide variety of natural bioactive compounds. Horizontal gene transfer is thought to play a role in the sporadic distribution of bioactive compounds producers among cyanobacteria. The most prevalent cyanobacterial natural products are produced in combinatorial pathways like mixed PKS/NRPS systems. Nonribosomal peptide synthetases (NRPSs), synthesize a diverse array of bioactive small peptides, many of which are used in medicine. Therefore, there is considerable interest in predicting NRPS substrate specificity in order to identification the natural bioactive products. To investigation the relationship between natural product genes and the role of horizontal gene transfer in the evolutionary history, we amplified and sequenced the NRPS genes. Moreover, we used some bioinformatics software tools, in order to prediction of peptide compound and an amino acid activated by a specific unknown NRPS A module by some cyanobacteria strains isolated from different Climatic/Geographic Regions and Habitats. Fischerella sp. Sara 1 KY618863, Nostoc sp. BN KY303912 and three Nostoc sp. isolated from agricultural areas, salt water and dry limestones, respectively, were analysed using a combination of the genome mining softwares, AntiSMASH, and NaPDoS. Moreover, Adenylation domain substrate specificity predictions for NRPS enzymes, the signature sequence and the name of the compound, were made using NRPSpreditor2. The results of bioinformatics analysis showed that, Microcistin synthetase B as natural compound, D A W F L G N V as signature sequences and McyB-M1-Leu as predicted amino acid were detected from Fischerella sp. Sara 1 KY618863, Nostoc sp. BN KY303912. However, NRPS gene fragments could not be amplified from three Nostoc sp. isolated from dry limestones. The negative PCR results from the initial NRPS PCRs were confirmed through subsequent annealing-temperature gradient NRPS PCRs. This result suggests that the ability of lateral gene transfer to produce the natural bioactive compound has been lost during evolutionary history in these strains. The data presented here highlight the need for future studies to define the linkages between Horizontal gene transfer in maintenance of NRPS genes among cyanobacteria distributed in different Climatic/Geographic Regions and Habitats. |
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| کلیدواژه‌های انگلیسی مقاله |
Prediction of substrate, Adenylation domains, NRPSs genes, Natural bioactive compounds, Horizontal gene transfer |
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| نویسندگان مقاله |
Fatemeh Taghavi zenuz - Department of Biology, East Tehran Branch, Islamic Azad University, Tehran
Reza Assareh - Young researched and elites clup Tehran north branch, Islamic Azad University. Tehran
Ali GhaderiKanzagh - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran
Bahareh Nowruzi - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran
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| نشانی اینترنتی |
www.icb7.ir |
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| زبان مقاله منتشر شده |
en |
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