|
|
نخستین کنفرانس بین المللی و دهمین کنگره ملی علوم باغبانی ایران
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژههای فارسی مقاله |
|
|
| عنوان انگلیسی |
The establishment of effective regeneration system and in vitro culture of Campanula |
|
| چکیده انگلیسی مقاله |
Several species of Campanula genus have been cultivated as ornamental plants, and there is an increasing demand for more attractive and novel characteristics. Optimal regeneration protocol is prerequisite for subsequent successful genetic manipulation and generation of new features. In current study the optimal media composition and explant source for shoot regeneration of three Campanula cultivars, C. portenschlagiana cvs. Blue Ocean (BO) and Royal; C. carpatica cv. Improved Blue Uniform (IBU) were determined. The focus of this study was based on avoiding over-supplementation of plant growth regulators (PGRs) especially cytokinins (TDZ). The explant which were examined for shoot regeneration were petioles harvested from two different explant sources; 1) Petioles harvested of three months old tissue-cultured plants, 2) Petioles harvested of etiolated nodal cuts. Furthermore various media compositions have been tested to find out the best composition for shoot regeneration of three cultivars and different NAA (0.5, 1.0 and 2.0 mg L-1) and TDZ (1.0, 2.0, 3.0 and 5.0 mg L-1) concentrations were investigated. According to the results, the most favorable explant source for harvesting petioles were nodal cuts of mature greenhouse plants. The best shoot regeneration obtained from the harvested petioles of nodal cuts in the three cultivars were, 85% IBU, 65% BO and 45.8% Royal. |
|
| کلیدواژههای انگلیسی مقاله |
shoot regeneration, explant source, TDZ concentration |
|
| نویسندگان مقاله |
Zeinab Ghayour karimani -
Margaret Serk -
|
|
| نشانی اینترنتی |
http://www.irhc2017.org |
| فایل مقاله |
دریافت فایل مقاله |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
|
| نوع مقاله منتشر شده |
|
|
|
|
برگشت به:
صفحه اول پایگاه |
دوره مرتبط |
کنفرانس مرتبط |
فهرست کنفرانس ها
|
